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Volume 70, Issue 1

gen. nov., sp. nov., a member of the family isolated from Asian honey bee gut, possesses a highly reduced genome Free

Abstract

The honey bee gut microbiota contains many bacterial lineages that are specific to this ecosystem. , raised across the Asian continent, is of great significance to the maintenance and development of ecology and agriculture in Asia. Here, we report the isolation and characterization of strain QZS01 from the gut of from Pingwu County, Sichuan Province, PR China. The results of phylogenetic analysis based on 16S rRNA sequences showed that strain QZS01 forms a monophyletic group together with clone sequences derived from variable insect hosts, and it shows 92% sequence similarity to its closest relative, . Strain QZS01 possesses a reduced genome (3.3 Mbp; G+C content, 38.05 mol%) compared to all other species, and the whole-genome based phylogenetic reconstruction showed that strain QZS01 represents a novel genus within the family . Strain QZS01 is a Gram-stain-negative facultative anaerobe. It grows on brain heart infusion agar and the energy sources utilized for growth are very limited. Based on the results of genotypic and phenotypic analyses, we propose a novel genus and species, gen. nov., sp. nov., with the type strain QZS01 (=CGMCC 1.13498=KCTC 62495).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Entomomonas , genome reduction , gut microbiota , honey bee and new genus
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31870472)
  • National Natural Science Foundation of China (Award 31772493)
© 2020 The Authors
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2019-09-26
2024-05-04
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Entomomonas moraniae gen. nov., sp. nov., a member of the family Pseudomonadaceae isolated from Asian honey bee gut, possesses a highly reduced genome
Int J Syst Evol Microbiol 70, 165 (2020); https://doi.org/10.1099/ijsem.0.003731
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